Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: Slices were stained using antibodies against: IFNAR1 (rabbit, Invitrogen, 1:100, PA5-120652), CD55 (rabbit, CST, 1:1200, 31759), EPCAM (rabbit, 1:200, CST, 93790), COX-2 (rabbit, 1:500, CST, 12282), F4/80 (rabbit, 1:200, CST, 70076), and CD31 (rabbit, 1:200, CST, 77699) overnight at 4°C.

Techniques: Expressing, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet:

Article Snippet: Slices were stained using antibodies against: IFNAR1 (rabbit, Invitrogen, 1:100, PA5-120652), CD55 (rabbit, CST, 1:1200, 31759), EPCAM (rabbit, 1:200, CST, 93790), COX-2 (rabbit, 1:500, CST, 12282), F4/80 (rabbit, 1:200, CST, 70076), and CD31 (rabbit, 1:200, CST, 77699) overnight at 4°C.

Techniques: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: Rabbit anti-CD55 antibody , CST , Cat# 31759; RRID: AB_2799010.

Techniques: Expressing, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet:

Article Snippet: Rabbit anti-CD55 antibody , CST , Cat# 31759; RRID: AB_2799010.

Techniques: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy

Journal: The FASEB Journal

Article Title: MAP‐2:CD55 chimeric construct effectively modulates complement activation

doi: 10.1096/fj.202300571r

Figure Lengend Snippet: FIGURE 1 Expression of the novel construct MAP-2:CD551-4. (A) Graphical representation of MAP-2 and MAP-2:CD551-4. (B–D) Western immunoblotting of the purified protein MAP-2:CD551-4 probed with mAb anti-MASP-2/Map19 (B), mAb anti-CD55 (C), and mAb anti-FLAG tag (D). rMAP-2 and rCD551-4 produced in-house were used as controls. (E) Direct protein stain of the same purified proteins.

Article Snippet: Membranes were blocked with 5% (w/v) skim milk (Sigma- Aldrich) for 30 min and then incubated with 0.5 μg/mL rat monoclonal antibody (mAb) anti- MASP- 2/ Map19, clone 6G12 (Hycult, Uden, The Netherlands), 1 μg/mL mouse mAb anti- CD55, clone 278803 (MAB2009; R&D Systems, Minneapolis, MN, USA), or 2 μg/mL mouse mAb anti- FLAG- tag (clone 18, produced in- house).

Techniques: Expressing, Construct, Western Blot, Purification, FLAG-tag, Produced, Staining

Journal: The FASEB Journal

Article Title: MAP‐2:CD55 chimeric construct effectively modulates complement activation

doi: 10.1096/fj.202300571r

Figure Lengend Snippet: FIGURE 2 Structural characterization of MAP-2:CD551-4. (A) MAP-2:CD551-4 elution profile from size exclusion chromatography exposed to 2 mM of calcium, 10 mM EDTA, or 10 mM EGTA. Vertical lines represent the molecular weight of the molecular marker, represented in kDa. (B–D) Western immunoblotting of the elution fractions 3–16 of MAP-2 from SEC exposed to 2.5 mM of calcium (B), 10 mM EDTA (C), or 10 mM EGTA (D). MAb anti MASP-2/Map19 clone 6G12 was used to detect MAP-2:CD551-4. Representative blots out of 3 independent replicates are shown. (E and F) Thermal stability of MAP-2, CD55, and MAP-2:CD551-4 exposed to 2 mM calcium or 10 mM EGTA shown as the ratio of the intrinsic fluorescence at 350 and 330 nm (E) and the right the first derivative of the ratio (F). Thermal stability was performed in triplicates.

Article Snippet: Membranes were blocked with 5% (w/v) skim milk (Sigma- Aldrich) for 30 min and then incubated with 0.5 μg/mL rat monoclonal antibody (mAb) anti- MASP- 2/ Map19, clone 6G12 (Hycult, Uden, The Netherlands), 1 μg/mL mouse mAb anti- CD55, clone 278803 (MAB2009; R&D Systems, Minneapolis, MN, USA), or 2 μg/mL mouse mAb anti- FLAG- tag (clone 18, produced in- house).

Techniques: Size-exclusion Chromatography, Molecular Weight, Marker, Western Blot, Fluorescence